Usage Guides
Step-by-step protocols for reconstituting, storing, and handling lyophilized research peptides.
Reconstitution Protocol
All Novex Research peptides ship as lyophilized (freeze-dried) powder. Reconstitution with bacteriostatic water (BW) is required before use in any aqueous research application.
What you need
Bacteriostatic Water
0.9% benzyl alcohol in sterile water. Preserves the peptide solution for up to 28 days at 2–8 °C. Do not use plain sterile water unless the solution will be used immediately.
Insulin Syringe (1 mL)
An insulin syringe with a fine-gauge needle (27–29 G) allows precise BW addition and minimizes vial septum damage.
Alcohol Swabs
Wipe both the BW vial top and the peptide vial septum with 70% isopropyl alcohol and allow to air-dry before inserting any needle.
Step-by-step
- Allow the peptide vial to reach room temperature (15–20 min) before opening.
- Draw the desired volume of bacteriostatic water into the insulin syringe.
- Wipe the septum of both the BW vial and the peptide vial with a fresh alcohol swab. Let dry.
- Insert the needle at a slight angle into the peptide vial. Run the BW slowly down the inside wall of the vial — do not jet it directly onto the powder.
- Gently swirl (do not shake or vortex) until the powder is fully dissolved. The solution should be clear and colorless or faintly yellow depending on the peptide.
- Label the vial with the reconstitution date and concentration (e.g., "BPC-157 1 mg/mL — 2026-02-25").
- Store reconstituted peptide in the refrigerator at 2–8 °C, away from light.
Never refreeze reconstituted peptide. Freeze-thaw cycles degrade the peptide chain and will reduce purity below the CoA-certified value.
Storage Guidelines
Lyophilized powder (sealed)
Stable at room temperature (below 25 °C) for up to 3 months when kept dry. For long-term storage (6–24 months), keep at −20 °C in a moisture-proof container. Avoid repeated temperature cycling.
Reconstituted solution
Refrigerate at 2–8 °C immediately after reconstitution. Use within 28 days. Keep away from light. Do not freeze the reconstituted solution.
General rules
Always store in a designated lab refrigerator/freezer — not a food fridge. Keep vials upright. Minimize the number of times a vial is punctured. Use sterile technique on every access.
Stability data for individual peptides may vary. Particularly sensitive compounds such as GHK-Cu and Selank should be stored at −20 °C even before reconstitution whenever possible. Check the CoA included with your order for compound-specific notes.
Recommended Equipment
The following equipment is typical for in-vitro peptide research. This list is for informational purposes and does not constitute an endorsement of any supplier.
Syringes & needles
1 mL insulin syringes (27–29 G) for reconstitution. 0.5 mL insulin syringes for precise small-volume transfers. Always use a new, sterile syringe for each vial access.
Bacteriostatic water
30 mL multi-dose vials of 0.9% benzyl alcohol in sterile water. Available from most lab supply vendors. Check expiration date before use.
0.22 µm syringe filter
Used to sterile-filter reconstituted peptide before use in cell-culture or in-vivo preclinical applications. Attach to a fresh syringe and push the solution through just before use.
Alcohol swabs
70% isopropyl alcohol prep pads for wiping vial septa and work surfaces. Use one swab per surface and allow to air-dry (≥30 seconds) before needle insertion.
Lab-grade storage container
A dedicated mini-freezer or insulated lab storage box at −20 °C for lyophilized stock. Label clearly with compound name, lot number, and date received.
PPE
Nitrile gloves, safety glasses, and a lab coat are minimum PPE for all peptide handling. Work in a laminar flow hood or biosafety cabinet if available.
Reading a Certificate of Analysis (CoA)
Every Novex Research product ships with a third-party CoA from an ISO 17025-accredited laboratory. A CoA is a formal quality document — here is how to read it.
Identity Confirmation
Mass Spectrometry (MS) confirms the molecular weight matches the theoretical MW of the stated compound. This rules out mislabeled or adulterated products.
Purity by HPLC
High-Performance Liquid Chromatography measures peak area as a percentage. Our minimum threshold is 98%; the majority of batches exceed 99%. Lower peaks represent synthesis byproducts.
Lot Number
Each CoA is tied to a specific production lot. The lot number on the vial should match the CoA. If they differ, contact support before use.
Test Date
CoAs are issued at time of manufacture. Peptide stability means the CoA values remain valid provided storage conditions outlined above are maintained.
CoA PDFs are linked directly on each product page. You can also email [email protected] with a lot number to request a CoA for any batch.
Handling & Safety
For in-vitro research use only. These compounds are not approved for human or veterinary use. Do not administer to any human or animal.
- Wear nitrile gloves and eye protection at all times when handling peptide vials or solutions.
- Work on a clean, non-porous lab surface wiped with 70% IPA before and after use.
- Dispose of needles and syringes in an approved sharps container. Never recap a used needle.
- Dispose of peptide solutions and empty vials according to your institution's chemical waste procedures.
- If skin or eye contact occurs, flush with large volumes of water for at least 15 minutes and contact your institution's safety officer.
- Do not eat, drink, or apply cosmetics in any area where peptides are handled.
- Keep all compounds locked and clearly labeled. Access should be restricted to qualified personnel.
Peptide Stability Reference
General stability estimates under proper storage conditions. Individual results may vary based on buffer composition, pH, and freeze-thaw history.
BPC-157
Lyophilized: stable 24+ months at −20 °C. Reconstituted: up to 28 days at 2–8 °C. Highly stable across pH 4–8.
TB-500 (Thymosin β4)
Lyophilized: stable 12–18 months at −20 °C. Reconstituted: 14–21 days at 2–8 °C. Sensitive to oxidation — minimize air exposure.
CJC-1295 / Ipamorelin
Lyophilized: stable 12–24 months at −20 °C. Reconstituted: up to 28 days at 2–8 °C. Relatively robust under standard lab conditions.
Semaglutide / GLP-1 analogs
Lyophilized: stable 18–24 months at −20 °C. Reconstituted: 28 days at 2–8 °C. Avoid repeated freeze-thaw. Protect from light.
GHK-Cu
Lyophilized: stable 12 months at −20 °C. Reconstituted: use within 14 days. More sensitive to oxidation than most peptides — store under inert conditions when possible.
Selank / Semax
Lyophilized: stable 12 months at −20 °C. Reconstituted: use within 14 days at 2–8 °C. Particularly sensitive to temperature fluctuation — avoid storage in door compartments of refrigerators.
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