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Research Guides

Usage Guides

Step-by-step protocols for reconstituting, storing, and handling lyophilized research peptides.

7 SectionsTopics covered
Step-by-stepProtocols
In-vitro onlyResearch use
01

Reconstitution Protocol

All Novex Research peptides ship as lyophilized (freeze-dried) powder. Reconstitution with bacteriostatic water (BW) is required before use in any aqueous research application.

What you need

Bacteriostatic Water

0.9% benzyl alcohol in sterile water. Preserves the peptide solution for up to 28 days at 2–8 °C. Do not use plain sterile water unless the solution will be used immediately.

Insulin Syringe (1 mL)

A fine-gauge needle (27–29 G) allows precise BW addition and minimizes vial septum damage.

Alcohol Swabs

Wipe both the BW vial top and the peptide vial septum with 70% isopropyl alcohol and allow to air-dry before inserting any needle.

Step-by-step

  1. 1Allow the peptide vial to reach room temperature (15–20 min) before opening.
  2. 2Draw the desired volume of bacteriostatic water into the insulin syringe.
  3. 3Wipe the septum of both the BW vial and the peptide vial with a fresh alcohol swab. Let dry.
  4. 4Insert the needle at a slight angle into the peptide vial. Run the BW slowly down the inside wall of the vial — do not jet it directly onto the powder.
  5. 5Gently swirl (do not shake or vortex) until the powder is fully dissolved. The solution should be clear and colorless or faintly yellow depending on the peptide.
  6. 6Label the vial with the reconstitution date and concentration (e.g., "BPC-157 1 mg/mL — 2026-02-25").
  7. 7Store reconstituted peptide in the refrigerator at 2–8 °C, away from light.
Never refreeze reconstituted peptide. Freeze-thaw cycles degrade the peptide chain and will reduce purity below the CoA-certified value.
02

Storage Guidelines

Lyophilized powder (sealed)

Stable at room temperature (below 25 °C) for up to 3 months when kept dry. For long-term storage (6–24 months), keep at −20 °C in a moisture-proof container. Avoid repeated temperature cycling.

Reconstituted solution

Refrigerate at 2–8 °C immediately after reconstitution. Use within 28 days. Keep away from light. Do not freeze the reconstituted solution.

General rules

Always store in a designated lab refrigerator/freezer — not a food fridge. Keep vials upright. Minimize the number of times a vial is punctured. Use sterile technique on every access.

Stability data for individual peptides may vary. Particularly sensitive compounds such as GHK-Cu and Selank should be stored at −20 °C even before reconstitution whenever possible. Check the CoA included with your order for compound-specific notes.

03

Recommended Equipment

The following equipment is typical for in-vitro peptide research. This list is for informational purposes and does not constitute an endorsement of any supplier.

Syringes & needles

1 mL insulin syringes (27–29 G) for reconstitution. 0.5 mL for precise small-volume transfers. Always use a new, sterile syringe for each vial access.

Bacteriostatic water

30 mL multi-dose vials of 0.9% benzyl alcohol in sterile water. Available from most lab supply vendors. Check expiration date before use.

0.22 µm syringe filter

Used to sterile-filter reconstituted peptide before use in cell-culture applications. Attach to a fresh syringe and push the solution through just before use.

Alcohol swabs

70% isopropyl alcohol prep pads for wiping vial septa and work surfaces. Allow to air-dry (≥30 seconds) before needle insertion.

Lab-grade storage container

A dedicated mini-freezer or insulated lab storage box at −20 °C for lyophilized stock. Label clearly with compound name, lot number, and date received.

PPE

Nitrile gloves, safety glasses, and a lab coat are minimum PPE for all peptide handling. Work in a laminar flow hood or biosafety cabinet if available.

04

Reading a Certificate of Analysis (CoA)

Every Novex Research product ships with a third-party CoA from an ISO 17025-accredited laboratory. A CoA is a formal quality document — here is how to read it.

Identity Confirmation

Mass Spectrometry (MS) confirms the molecular weight matches the theoretical MW of the stated compound. Rules out mislabeled or adulterated products.

Purity by HPLC

High-Performance Liquid Chromatography measures peak area as a percentage. Our minimum threshold is 98%; the majority of batches exceed 99%.

Lot Number

Each CoA is tied to a specific production lot. The lot number on the vial should match the CoA. If they differ, contact support before use.

Test Date

CoAs are issued at time of manufacture. Peptide stability means CoA values remain valid provided storage conditions outlined above are maintained.

Every Novex Research order ships with the CoA for your batch. You can also email [email protected] with your lot number to request a copy at any time.

05

Handling & Safety

For in-vitro research use only. These compounds are not approved for human or veterinary use. Do not administer to any human or animal.
  • Wear nitrile gloves and eye protection at all times when handling peptide vials or solutions.
  • Work on a clean, non-porous lab surface wiped with 70% IPA before and after use.
  • Dispose of needles and syringes in an approved sharps container. Never recap a used needle.
  • Dispose of peptide solutions and empty vials according to your institution's chemical waste procedures.
  • If skin or eye contact occurs, flush with large volumes of water for at least 15 minutes and contact your institution's safety officer.
  • Do not eat, drink, or apply cosmetics in any area where peptides are handled.
  • Keep all compounds locked and clearly labeled. Access should be restricted to qualified personnel.
06

Peptide Stability Reference

General stability estimates under proper storage conditions. Individual results may vary based on buffer composition, pH, and freeze-thaw history.

BPC-157

Lyophilized: stable 24+ months at −20 °C. Reconstituted: up to 28 days at 2–8 °C. Highly stable across pH 4–8.

TB-500 (Thymosin β4)

Lyophilized: stable 12–18 months at −20 °C. Reconstituted: 14–21 days at 2–8 °C. Sensitive to oxidation — minimize air exposure.

CJC-1295 / Ipamorelin

Lyophilized: stable 12–24 months at −20 °C. Reconstituted: up to 28 days at 2–8 °C. Relatively robust under standard lab conditions.

Semaglutide / GLP-1 analogs

Lyophilized: stable 18–24 months at −20 °C. Reconstituted: 28 days at 2–8 °C. Avoid repeated freeze-thaw. Protect from light.

GHK-Cu

Lyophilized: stable 12 months at −20 °C. Reconstituted: use within 14 days. More sensitive to oxidation — store under inert conditions when possible.

Selank / Semax

Lyophilized: stable 12 months at −20 °C. Reconstituted: use within 14 days at 2–8 °C. Sensitive to temperature fluctuation — avoid door compartments of refrigerators.